"Are you really trying to create a high throughput electron microscopy workflow?"
Yes. We've increased acquisition rates by a factor of ~15-20x over what is available using commercially available TEM systems. We now have tens of terabytes of image data in the can, and when I'm not reading HN (argh!) I'm working on collating & analyzing these data.
Light level microscopy preceded embedding of the material for EM, the anatomy is correlated between the two modalities, and the sample preparation method (this is of mouse brain) is by perfusion with an aldehyde mixture, so there is essentially no deterioration of the ultrastructure.
So, I wonder what you do -- no home page in your profile -- but I do remember what it's like to make a living. I like biology better. :)
I used to work in a bioinformatics startup - tons of fun with cutting edge science. But the startup tanked, and I moved on to a high paying corporate software engineering job. But I hope to be back in bioinformatics before long.
Thanks. :) It's been a long road, hope to get a paper out in the next ~6 months or so. Shoot me an email (address on my home page) if you ever want to be in touch outside of HN.
Yes. We've increased acquisition rates by a factor of ~15-20x over what is available using commercially available TEM systems. We now have tens of terabytes of image data in the can, and when I'm not reading HN (argh!) I'm working on collating & analyzing these data.
Light level microscopy preceded embedding of the material for EM, the anatomy is correlated between the two modalities, and the sample preparation method (this is of mouse brain) is by perfusion with an aldehyde mixture, so there is essentially no deterioration of the ultrastructure.
So, I wonder what you do -- no home page in your profile -- but I do remember what it's like to make a living. I like biology better. :)