It makes a lot of sense in retrospect because all the structures are visible on the brightfield image - it's just more annoying for a human being to pick out the features on thousands of slides.
I wonder what the dye budgets and spectral bands will go to now that there is no need to mark the boundary of the nucleus. I bet there are a lot of things that are not visible in the refractive index that you could dye.
I wonder what the dye budgets and spectral bands will go to now that there is no need to mark the boundary of the nucleus. I bet there are a lot of things that are not visible in the refractive index that you could dye.