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As the fellow states in his video, he was limited by the capabilities of the microscope. The quality of images he took required 10 seconds per frame. The microscope was capable of producing something much blurrier at 60 fps.

You would need to record several thousand frames per second to produce something other than a blur when looking at a record producing audio in real-time. Human hearing extends up above 20 KHz and vinyl records are designed to handle this. Recording a normal object at tens of thousands of frames per second would require a very expensive camera and intense lighting. Doing this through an optical microscope would be substantially more difficult, and a current world class electron microscope would probably be incapable of such frame rates.

The scale of a vinyl record groove and needle suggests that a good optical microscope would be sufficient. However, this appears to be a case of a fun project chosen to test out a tool, rather than a tool chosen specifically for the project.

Maybe you could use a strobe effect: Have an audio sample that loops every 1/100th of a second, and then put a "fan" spinning at the same rate in front of the electron source, which blocks the electrons except for 1/60th or less of the 1/100th of a second. As you vary the phase of the fan, you capture the needle at different positions in the loop.

That would have been possible if he were able to observe the vinyl in action and had a circular track of audio on the record, instead of working with a square slice.

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