A form of virtualization was first demonstrated with IBM's CP-40 research system in 1967, then distributed via open source in CP/CMS in 1967–1972, and re-implemented in IBM's VM family from 1972 to the present. Each CP/CMS user was provided a simulated, stand-alone computer.
This is incidentally how Windows 386-9x ran DOS applications - in a VM, using V86 mode.