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That what lab assistant usually says. At uni we used some blue florescent dye that apparently got ionically bonded to P group of DNA backbone. It was completely safe(not carcinogenic). I can't remember the name of the dye, but results with EtBr were much clearer.



What about Methylen blue? Do you guys have an idea if this will work? Read it somewhere. But i don't think it is florescent.

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If my memory doesn't lie to me, I think its a load dye, which only dyes the DNA sample to visually assist during the loading of the samples into the "wells" of the gel. Otherwise you can't be sure whether you put it inside the well or somewhere into the surrounding buffer.

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GelRed is another safe one. It is designed so that it will not cross cell membranes. I don't think it intercalates either.

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