1. In vitro petri dish testing is a million light years away from showing a compound works in humans.
2. This is a particularly bad problem in brain diseases as getting your compound to cross the blood-brain barrier in correct therapeutic quantities is extremely difficult.
3. We have lots of direct antibodies that kill beta amyloid in vivo that are very advanced in human trials. The problem now is that they often cause micro-damage when removing various forms of amyloid protein or have been shown to be administered far too late when the damage is already done.
4. It's still not clear that beta amyloid is even the main causal agent of Alzheimers!
In general, if you see someone touting a low effort, pre-clinical study as major medical breakthrough, start hitting the downvote button. I'm disappointed this even got to the main page.
Maybe its a disease caused by glial cell dysfunction where beta amyloid is just the most obvious but not most important piece of trash that collects in the brain. And it's just a terrible coincidence injections of beta amyloid have similar effects as Alzheimer's.
It's well-established THC crosses the blood brain barrier due to its mental effects. Here are some more evidential links to support this:
It's also really useful when there's comment on Hacker News pointing out the things that have been glossed over in an over-hyped article, so thanks. (no sarcasm intended)
I know this will get downvoted but it's too bad there's no downvote button on HN
Edit: although stories can't be downvoted.
which is based on this press release:
original paper here:
"Amyloid proteotoxicity initiates an inflammatory response blocked by cannabinoids"
"But THC can also bind to them in much the same way, and when they do, they start messing with your brain’s ability to communicate with itself. The [SIC] can be a good and a bad thing, because while you might forget something important or suddenly be incapable of swinging a baseball bat, you’ll probably feel amazing, and want to eat all the snacks:"
http://www.sousweed.com/blog/2015/6/13/sous-vide-cannabis-bu... is a recipe I've followed with great results.
Edit: Plus now you can sous vide meats and whatever. It's a win-win.
If I was going to experiment with a new extraction method, my choice would be to acquire a soxhlet extractor, and experiment with ethanol as a solvent, for producing budder. https://www.erowid.org/archive/rhodium/pdf/soxhlet4dummies.p..., page 2 shows a DIY version that doesn't require you to purchase glassware. I would recommend putting the whole setup in a larger pot of water, so that you can more easily control the temperature. You can use the same setup as a still, to extract ethanol from something like cheap vodka.
As for methods that I have actually tried, cold butane extraction produces spectacularly clear oil, which eventually dries to shatter. Do freeze the weed first, don't freeze your hand. Do leave the extract spread out in a thin layer to evaporate until it forms hard shatter, otherwise some very scary butane will remain in the product.
It's great if researchers can find new treatments, but I think people get over-enthusiastic with medical cannabis
> Anyone have some experience with this type of gear?
I would rather vaporize weed. Very simple and (likely) safe, effect is immediate and it's easier to adjust the quantity as you go.
I'm not sure about the gear but I cooked cannabis butter a few times (quite easy without special equipment). It's more difficult to control the quantity. You need to be careful to not ingest too much cannabis, because then it's too late and it can be very powerful. Conversely, if not enough, you have to wait for an hour or so to be sure. Another issue I had is that I ended up with a nice cake that I had to ration carefully as it was very psychoactive. I couldn't simply eat it whenever I felt like. Besides, the weed doesn't add a good taste (at best neutral, depending on the quantity).
I'm sure that these issues can be overcome with some experience but is it worth the trouble?
Pure acetone (no colorants or scents added) and a hot plate is all you need. Acetone wash your ground material for 10 seconds, filter/strain through coffee filters onto a hot plate set at ~150F (make sure this is in a well-ventilated room) and let evaporate for an hour.
Done and ready to go. Far safer than most other extraction methods, which rely upon high gas pressures or even more highly-flammable (and explosive) substances.
Saturated unsubstituted hydrocarbons (e.g. hexane) are really nice to work with, e.g. provide similar results to butane at room temperature/pressure. Heptane is nice because it can in principle be "natural" if sourced from Jeffrey pine oil, but I haven't seen that for sale...
This is why you've got it on the hot plate for an hour - at 150F the acetone's gone in 20 minutes, you need the other 40 minutes to purge the water from the extract.
Heptane is also about 6x the price of acetone.
There haven't been enough studies to actually prove this. Most show an issue while being high (and many studies combine marijuana usage with alcohol), but nothing that directly shows negative long-term effects. If this study is held up by their further human trials they mention, then it would indicate that pop-culture is probably wrong.
* Marijuana causes short-term short-term memory loss, but doesn't cause long-term short-term memory loss.
Unfortunately I can't find the study info now (@ work, so I'm kinda limited on spending too much time research pot...)
It isn't like "big pharma" won't make money off of it, since they'll be able to grow their own crops, complete with GMO help to produce more of the "helpful" stuff, afterwards concentrating it into pill or inhaler form and checking for purity. The GMO and the final pill can likely be patented, giving them reason to pressure the government by lobbying. Something similar with health insurers, as lowering costs is in their best interests since Alzheimer's can be an expensive disease.
The main lobby they'd have to worry about in the US is from places like private prisons and the like, because they can lose money with this - but even that is doubtful unless they change the laws for recreational use.
Additionally, public opinion has been increasingly changing for this sort of thing, and that is starting to be shown through government policies (though slowly with the current administration's attitude), as evidenced by the fast-tracking of MDMA trials treating PTSD. I'm not sure that could have happened 20 years ago, and definitely not 30 years ago during the "Just say no!" heyday.